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KMID : 0374919960170020229
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Inje Medical Journal
1996 Volume.17 No. 2 p.229 ~ p.234
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Protoplast Fusion of Lactobacillus acidophilus
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Abstract
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The optimal conditions for the formation and regeneration of Lactobacillus acidophilus protoplasts were exmined. Protoplast formation of L. acidophilus was most efficiently obtained when the cells grown to 6-10 h in MRS medium were used. The
maximum
number(98%) of protoplasts was obtained when lysozyme was treated for 45 min to lysis the cell wall in 20 mM HEPES buffer (pH 7.0) containing 1.0 M sucrose and in the rage of pH 6.0-7.0. maximal regenerated protoplasts were obtained o complex
medium
containing 10% sucrose, 6 mm MgCl2 and 2.5% gelatin. The regeneration frequency of the protoplasts was ca. 4.5% after 3 days of incubation at 37℃.
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